Chlamydia trachomatis is the most common cause of nongonococcal urethritis in the world. This is detected by several methods: direct staining methods (Iodine, Giemsa, Direct immunofluorescence stain), serologic methods (ELISA, indirect immunofluorescence), Culture methods (Yolk sac, McCoy cell, HeLa cell, Lister cell culture). The standard method in isolation of Chlamydia trachomatis is the vial culture using monolayer of McCoy cells, but this method is not proper to mass screening for public health because this method was to time consuming and no economic. So we have tried to develop the massive sereening culture method using 96 well microplate in Korea. Clinical specimens were collected from urethral mucosa´of 142 male health students who were volunteer in this study. This specimens were inoculated into cycloheximide-treated McCoy cell monolayer in 96 well microplate and incubated in the CO2 inoculator (37C) for 48 and 72 hours. We used iodine stain for detection of Chlamydia trachomatis.
The summaried results were following.
1. The positive rates of Chlamydia trachomatis in the 48 hour and 72 hours culture were 7.7% and 7.0% in respective and totally positive rate of 43 hours or 72 hours were 10.6%
2. The positive rates of Chlamydia trachomatis according to sample storage were decreased.
I: within 7 days (15.8%) II: 914 days (9.9%) III: 15---21 days (14.2%) IV: 2228 (6.6%) V: more 29 days (6.3%)
In summarized our study results, the reserve rate of Chlamydia trachomatis in the health male (students) were very high (10.6%) and the culture method using 96 well microplate was very easy and economic in Korea.
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